DICIEMBRE 2009 N° 10 Volumen 7

Comparison of two diagnostic methods for detecting infection in chronic wounds


Contact address

Teresa Segovia Gómez. Unidad de Úlceras por Presión y Heridas Crónicas. Hospital Universitario Puerta de Hierro Majadahonda. c/ Joaquín Rodrigo, 2. 28

Contact email: tsegovia.hpth@salud.madrid.org


Objective: to ascertain the degree of concordance in microbiological diagnosis of infections in chronic wounds between two sample collection methods: by swab and by aspiration.
Methods: prospective observational study comparing two diagnostic methods, the culture by swab method” versus the culture by aspiration method in the detection of infection in a cohort of patients with chronic wounds treated at the Multidisciplinary Pressure Ulcers and Chronic Wounds Unit, University Hospital Puerta de Hierro Majadahonda (HUPHM), Madrid. In all cases (patients >18 years of age, with evident infection in chronic wound, with a suspected chronic wound infection or diabetic foot) the procedure for obtaining samples for culture and successive samples were conducted in the same way: by expert nurses from the Pressure Ulcers and Chronic Wounds Unit, following standard protocols. Primary variable: growth in culture of specific microorganisms. Data Collection and analysis: Data collection book, patient clinical history, laboratory results (microbiological, biochemical, hematological...) and physical examination. Statistical analysis was conducted for each specific organism by comparing tests of concordance between both methods of sample collection.
Applicability: the generation of new evidence in this area will create a strong case for developing clinical practice guidelines on monitoring and detection of infection in chronic wounds.


chronic wound; ulcer; infection; Nursing diagnosis; research project

Versión en Español


Comparación de dos métodos diagnósticos en la detección de infección en las heridas crónicas